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Oxygen Content Testing


Alfa Chemistry provides services to test graphene's oxygen content. Graphene oxide is rich in various oxygen-containing groups such as hydroxyl, epoxy and carboxyl groups, and is the precursor of many functionalized graphite derivatives.Alfa Chemistry uses a variety of methods to detect the oxygen group and oxygen content in raw graphene and the devices made from it.

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Our Instrument Platforms for Oxygen Content Analysis


  • UV Visible Spectrometer

UV-Vis spectroscopy can be used for qualitative and quantitative analysis of graphene. The ultraviolet spectrum of the obtained product is compared with the ultraviolet spectrum of graphene or its derivatives to determine whether the obtained product is graphene or its derivatives.

Oxygen-Content-PictureFigure 1. UV-vis spectra of graphene oxide and graphene. (Wazir A.H, et al, 2016)

  • FTIR Spectrometer 

The main purpose of FTIR spectrum is to determine the type and content of functional groups. The uniqueness of this technology is that it is a collection of absorption bands, which helps to confirm the identity of pure compounds or detect the presence of specific impurities. The oxygen-containing group content can be calculated according to the position, intensity and peak area of the absorption peak of the oxygen-containing functional group in the FTIR spectrum, combined with the relevant infrared spectrum calculation formula.

Oxygen-Content-PictureFigure 2. FTIR spectrum of graphene oxide and graphene. (Çiplak Z, et al, 2015)

  • X-ray Photoelectron Spectroscopy

X-ray photoelectron spectroscopy (XPS) technology can be used to analyze the carbon to oxygen ratio in graphene and its derivatives. XPS is used to analyze graphene through an oxygen functionalization procedure. In addition, XPS does little damage to the sample, which is very useful for materials research.

  • Thermogravimetric Analyzer

Testing thermal stability is also one of the methods to study the existence of oxygen-containing functional groups by studying the degradation of samples. Graphite is a thermally stable compound,. After oxidation, the graphite structure changes drastically to CGO and contains a large number of oxygen-containing functional groups. The decomposition temperature of graphene oxide is 200 ℃. If the graphene oxide is reduced, that is, oxygen is removed from the surface, its decomposition temperature will increase, and the temperature increase depends on the reducing agent used. The thermogravimetric analysis of graphite, graphene oxide and reduced graphene is usually performed using a thermogravimetric analyzer under nitrogen flow.

Oxygen-Content-PictureFigure 3. Thermogravimetric results of graphene oxide samples. (Aliyev E, et al, 2019)

Detectable Samples Include:

Graphene, graphene oxide, reduced graphene oxide, nanographene and etc.

Testing Standards

  • GB/T 30544.13-2018 ISO/TS 80004-13:2017(en): Graphene and Related Two-dimensional (2D) Materials.
  • ASTM C562-1991(2005) Method for Testing Moisture in Graphite Samples.

Why Us?

1. Laboratory management system in line with ISO/IEC17025 international standards.

2. A variety of analysis and testing tools.

3. High-level, professional and experienced technical team.

4. The detection cycle is short and the experiment procedure is perfect.


  1. Wazir A.H, et al. (2016). "Synthesis of Graphene Nano Sheets by the Rapid Reduction of Electrochemically Exfoliated Graphene Oxide Induced by Microwaves." J Chem Soc Pakistan. 38(1), 11-16.
  2. Çiplak Z, et al. (2015). "Investigation of Graphene/Ag Nanocomposites Synthesis Parameters for Two Different Synthesis Methods." Fullerenes, Nanotubes and Carbon Nanostructures. 23(4), 361-370.
  3. Aliyev E, et al. (2019). "Structural Characterization of Graphene Oxide: Surface Functional Groups and Fractionated Oxidative Debris." Nanomaterials (Basel). 9(8), 1180.
Our products are for research use only and cannot be used for any clinical purposes.