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Della Pelle, Flavio, et al. Chemistry-A European Journal 22.36 (2016): 12761-12766.
For the first time, a carbon black nanoparticle (CBNP) pressure-transfer film-based transducer for microscale molecular detection was proposed. Current sensing atomic force microscopy (CS-AFM) showed that the CBNP film was effectively pressure-transferred and maintained good conductivity.
Preparation of CBNP Films
A dispersion of CBNP in 1,2-dichloroethane (0.50 mg/10 ml) was used. The dispersion was obtained by using a bath sonicator for 15 minutes followed by tip sonication for 5 minutes.
The pressure transfer membrane was obtained by using the following steps:
a) Filtration was performed using an analytical stainless steel 13 mm vacuum filter holder. The CBNP dispersion (3 to 20 mL) was filtered under vacuum by using a Teflon filter with a pore size of 0.1 µm;
b) The Teflon filter was cut into pieces of 13 x 1 mm2 to obtain the CB wire;
c) The CB wire was placed on polymethyl methacrylate and 1,2-dichloroethane (1 µL) was deposited on it;
d) A polyethylene terephthalate sheet (3.4 x 1.0 x 2 mm3) cleaned with ethanol and deionized water was placed above the polymer;
e) The CB was transferred to the sheet by applying a pressure of (5.8 ± 0.1) tons for 60 seconds. The Teflon filter was removed using tweezers;
f) Electrical contact was made by using conductive silver which was subsequently electrically isolated by using an insulating coating;
g) The CBNP membrane was coupled for microchip detection.
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